Purification and properties of ornithine racemase from Clostridium sticklandii.
نویسندگان
چکیده
Ornithine racemase has been purified to homogeneity from Clostridium sticklandii, as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This is the first racemase known to be highly specific to ornithine. This PLP-dependent enzyme has an M(r) of 92, 000, with a K(m) for L-ornithine of 0.77 +/- 0.05 mM and a k(cat) of 980 +/- 20 s(-1).
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عنوان ژورنال:
- Journal of bacteriology
دوره 182 7 شماره
صفحات -
تاریخ انتشار 2000